Primary structure of microbial transglutaminase from Streptoverticillium sp. strain s-8112
نویسندگان
چکیده
منابع مشابه
Primary structure of microbial transglutaminase from Streptoverticillium sp. strain s-8112.
The complete amino acid sequence of transglutaminase (EC 2.3.2.13) (TGase), which is produced by a microorganism, Streptoverticillium sp. strain s-8112, and catalyzes the acyl transfer reaction between gamma-carboxyamide groups of glutamine residues in proteins and various primary amines, has been established by a combination of fast atom bombardment mass spectrometry and standard Edman degrada...
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A “ready-to-use” preparation for the production of frankfurter type sausage („Activa WM“, AJINOMOTO Europe Sales GmbH, Hamburg) was used for the studies. Protein was determined by a modified method according to Bradford [1]. Results were calculated using the absorbance quotient of A590nm and A450nm. SDS polyacrylamide gel electrophoresis (SDS-PAGE) was carried out according to Laemmli [2] on a ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1993
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)50238-1